WT1--EWSR1

Hyperlinks to databases below
COSMIC CHIMERKB CHIMERPUB CHIMERSEQ CHITARS
FARE-CAFE TICDB TUMOR_FUSION_GDPFusionCancerConjoinG
1000Genome18CancersBodymap2HPANon_Tumor_Cells
Babiceanu_DatasetBanned_DatasetKnown_FusionsONGene DatabaseBushman Cancer Gene Database
Tumor Gene Set By UniprotOesophagus_DatasetGliomas_DatasetProstate_DatasetPancreases_Dataset
GTExKlijn_DatasetFimereli_Dataset Literature Cortex_Dataset
ChromothripsisDB

Link to Genecards:

WT1 EWSR1

COSMIC

The fusion gene pair WT1--EWSR1 information is not available in COSMIC database.

ChimerKB

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The fusion gene pair WT1--EWSR1 information is available in CHIMERKB (CHIMERDB 3.0) database.

Fusion_pair5'Gene Junction (Chr/Position/Strand)3'Gene Junction (Chr/Position/Strand)Breakpoint_TypeGenome_BuildDiseaseValidationPMIDGene TypeSource
WT1_EWSR1-/-/ -/-/ - hg18 small round cell tumor - 7862627 - OMIM

ChimerPUB

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The fusion gene pair WT1--EWSR1 information is available in CHIMERPUB (CHIMERDB 3.0) database.

Fusion_pairTranslocationPMIDDiseaseValidationGene TypeSentence_highlight
WT1_EWSR1- 25454478glomus tumor FISH - Subsequent molecular analysis showed the presence of an EWSR1-WT1 gene fusion by fluorescence in situ hybridization, which was confirmed by reverse- transcriptase polymerase chain reaction. /// "To our knowledge, this is the first case reported with EWSR1-WT1 fusion in a small round blue cell tumor with smooth muscle differentiation and an indolent course.. " /// Low-grade small round cell tumor of the cauda equina with EWSR1-WT1 fusion and indolent clinical course..
WT1_EWSR1t(11;22)(p13;q12) / 24388397- FISH - Multiple EWSR1-WT1 and WT1-EWSR1 copies in two cases of desmoplastic round cell tumor.. /// Our study demonstrated that an integrated molecular cytogenetic approach identified EWSR1-WT1 cooperating molecular events and genetic markers for prognosis. /// "Common genomic events (i.e., trisomy 3 and extra EWSR1-WT1 and WT1-EWSR1 copies) probably contributed to disease pathogenesis and/or evolution of DSRCT. "
WT1_EWSR1t(11;22) / 24953786desmoplastic small round cell tumor PCR;RT-PCR - Fluorescence in situ hybridization studies using an EWSR1 break-apart probe confirmed the presence of a rearrangement involving the EWSR1 locus and RT-PCR demonstrated the presence of an EWSR1-WT1 fusion transcript associated with the t(11;22) rearrangement, which supported a diagnosis of DSRCT. /// "Fluorescence in situ hybridization studies using an EWSR1 break-apart probe confirmed the presence of a rearrangement involving the EWSR1 locus and RT-PCR demonstrated the presence of an EWSR1-WT1 fusion transcript associated with the t(11;22) rearrangement, which supported a diagnosis of DSRCT."
WT1_EWSR1t(11;22)(p13;q12) / 22587803ewing sarcoma;sarcoma FISH;PCR;RT-PCR - Using COBRA-FISH following a metaphase approach we demonstrated a balanced translocation, t(11;22)(p13;q12) and in RT-PCR formation of the EWSR1-WT1 fusion product, a specific translocation of desmoplastic round cell tumour. /// The EWSR1-WT1 chimera seems to function as an oncogenic transcription factor.
WT1_EWSR1t(11;22)(p13;q12) / t(1;15) / 25133149desmoplastic small round cell tumor - - Cytogenetic studies showed translocation t(11;22)(p13;q12) confirming an EWSR1-WT1 translocation characteristic for DSRCT, and t(1;15)(q11;p11.2) of unknown significance.
WT1_EWSR1- 21987447osteosarcoma PCR;ISH - We report the case of a 12-year-old girl with multifocal small cell osteosarcoma positive for the Ewing sarcoma breakpoint region 1 (EWSR1) gene rearrangement by interphase fluorescent in situ hybridization and negative for EWSR1-FLI1, EWSR1-ERG, and EWSR1-WT1 fusion transcripts by reverse transcriptase PCR.
WT1_EWSR1- 24832162nephroblastoma - - However, focal triphasic elements and glomeruloid bodies were identified on deeper sections, WT1 immunoreactivity was seen with antibodies to both the amino-terminus and carboxy-terminus, and EWSR1-WT1 rearrangement studies were negative, supporting the ultimate diagnosis of WT. /// "In these challenging cases, detection of an EWSR1-WT1 rearrangement and selective WT1 carboxy-terminus immunoreactivity (characteristic of DSRCT) or dual immunoreactivity for the WT1 amino-terminus and carboxy-terminus (characteristic of WT) remain the most discriminating diagnostic tools. "
WT1_EWSR1- 27180056desmoplastic small round cell tumor FISH;PCR;RT-PCR - Three cases (1.5%) revealed the SYT-SSX transcript for Synovial sarcoma, and one (0.5%) a EWSR1-WT1 transcript for Desmoplastic Small Round Cell tumor. /// "All tumors were tested for EWSR1-ETS, EWSR1/WT1, PAX3/7-FOX01 or SYT/SSX transcripts, and the negative tumors were subsequently analyzed for CIC/DUX4, BCOR/CCNB3 and CIC/FOX04 transcripts. "

ChimerSEQ

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The fusion gene pair WT1--EWSR1 information is not available in CHIMERSEQ (CHIMERDB 3.0) database.

ChiTaRS 2.1

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The fusion gene pair WT1--EWSR1 information is not available in CHITARS database.

FARE-CAFE

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The fusion gene pair WT1--EWSR1 information is available in FARE-CAFE.

Cancer Information
Fusion_ProteinCancer_TypeFP_Experimental_MethodFP_KaryotypeFP_Reference
WT1-EWSR1 Desmoplastic Small Round Cell Tumor Southern blot,RT-PCR,DNA sequencing,PCR t(11;22)(p13;q12) PubMed:7862627

Genomic Information
Fusion_ProteinFusion_Protein_Isoforms5'_ProteinF_B_PFBP_in_Exon/Intron5'_Protein_on_Positive/Negative_Strands3'_ProteinS_B_P3'_Protein_on_Positive/Negative_StrandsSBP_in_Exon/IntronFusion_Protein_mRNA_sequenceFusion_Protein_ReferenceFusion_Protein_GenbankID
WT1-EWSR1 WT1-EWSR1-Isoform1 WT1 _ _ 0 EWSR1 _ 1 _ _ _ _
WT1-EWSR1 WT1-EWSR1-Isoform2 WT1 _ _ 0 EWSR1 29683104 1 1 _ _ _

Domains and DDI Information
Fusion_ProteinFusion_Protein_IsoformsDomains_in_5'PartnerDDIs_for_Domains_in_5'PartnerDomains_in_3'PartnerDDIs_for_Domains_in_3'Partner5'_Protein5'P_Domains5'P_Domains_DDI_partners3'_Protein3'P_Domains3'P_Domains_DDI_partnersMissing_FP_DomainsMissing_FP_Domains_DDI
WT1-EWSR1 WT1-EWSR1-Isoform1 - - - -WT1 1) WT1
2) zf-H2C2_2
1) zf-C2H2 , zf-H2C2_2 , zf-C2H2_4 , zf-C2H2_6
EWSR1 1) RRM_6
2) zf-RanBP
1) RRM_6 , RRM_5 , RRM_1 , NUDIX_2 , Vinculin , zf-RNPHF
2) ubiquitin , Ras
1) WT1
2) zf-H2C2_2
3) RRM_6
4) zf-RanBP
1) zf-C2H2 , zf-H2C2_2 , zf-C2H2_4 , zf-C2H2_6
2) RRM_6 , RRM_5 , RRM_1 , NUDIX_2 , Vinculin , zf-RNPHF
3) ubiquitin , Ras
WT1-EWSR1 WT1-EWSR1-Isoform2 - - 1) RRM_6
2) zf-RanBP
1) RRM_6 , RRM_5 , RRM_1 , NUDIX_2 , Vinculin , zf-RNPHF
2) ubiquitin , Ras
WT1 1) WT1
2) zf-H2C2_2
1) zf-C2H2 , zf-H2C2_2 , zf-C2H2_4 , zf-C2H2_6
EWSR1 1) RRM_6
2) zf-RanBP
1) RRM_6 , RRM_5 , RRM_1 , NUDIX_2 , Vinculin , zf-RNPHF
2) ubiquitin , Ras
1) WT1
2) zf-H2C2_2
1) zf-C2H2 , zf-H2C2_2 , zf-C2H2_4 , zf-C2H2_6

miRNA Information
Fusion_ProteinmiRNAs_Targets_Fusion_proteinMissing_MiRNAs_Targets_Fusion_proteinFP_miRNA_Validation_method
WT1-EWSR1 hsa-let-7a-5phsa-miR-877-3phsa-miR-615-3phsa-miR-423-3phsa-miR-328-3phsa-miR-362-5phsa-miR-320ahsa-miR-149-5phsa-miR-23b-3phsa-miR-196a-5phsa-miR-100-5phsa-let-7c-5p hsa-miR-16-5phsa-miR-15a-5phsa-miR-212-3p Report assay,Western Blot,Microarray,qPCR,NGS

Transcription Factors Information
Fusion_Protein5'P_Ref_mRNA_seqIDFP_Transcription_factorsFP_Missing_TFsFP_TF_Reference
WT1-EWSR1 NM_000378.4 1) PAX8_HUMAN
2) TRIM28_HUMAN
3) STAT1_HUMAN
4) P53_HUMAN
5) RUNX1_HUMAN
6) ETS1_HUMAN
7) EGR1_HUMAN
8) FOS_HUMAN
9) GATA2_HUMAN
10) E2F1_HUMAN
11) TAL1_HUMAN
12) CTCF_HUMAN
13) BRG1_HUMAN
14) HNF4A_HUMAN
15) FOXA1_HUMAN
16) CEBPA_HUMAN
- 1) 9388203
2) 17542650
3) 19122651
4) 21394211
5) 20019798
6) 20019798
7) 20690147
8) 20139302
9) 21808000
10) 21310950
11) 21795385
12) 21795385
13) 19505939
14) 21646355
15) 21572391
16) 20219941

TicDB

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The fusion gene pair WT1--EWSR1 information is not available in TicDB.

TUMOR FUSION Gene Data Portal

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The fusion gene pair WT1--EWSR1 information is not available in TUMOR FUSION Gene Data Portal.

FusionCancer

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The fusion gene pair WT1--EWSR1 information is not available in FusionCancer Database.

ConjoinG

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The fusion gene pair WT1--EWSR1 information is not available in ConjoinG Database.

1000Genome

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Fusion gene WT1--EWSR1 has not been seen in a healthy sample (RNA-seq data from some samples from 1000 genomes project: Greger et al., Tandem RNA Chimeras Contribute to Transcriptome Diversity in Human Population and Are Associated with Intronic Genetic Variants, Plos One, Aug 2014 ). Therefore this candidate fusion gene has a low probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

18Cancers

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Fusion gene WT1--EWSR1 is not found in a RNA-seq dataset of 18 types of cancers from 600 tumor samples (B. Alaei-Mahabadia et al., Global analysis of somatic structural genomic alterations and their impact on gene expression in diverse human cancers, PNAS, Nov. 2016 )

Bodymap2

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Fusion gene WT1--EWSR1 is not found in the list of known false positive fusion genes. The list has been generated from healthy human samples collected from 16 organs from Illumina BodyMap2 RNA-seq database. A candidate fusion gene found in this list has a very high probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

HPA

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Fusion gene WT1--EWSR1 is not found in a healthy sample (RNA-seq database of 27 healthy tissues from 95 human individuals). A candidate fusion gene found in this dataset has a very high probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

Non_Tumor_Cells

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Fusion gene WT1--EWSR1 was not found among the fusion genes which have been previously reported/found in non-tumor cell lines, like for example HEK293. The genes which are observed in those list can be considered as non-somatic mutation. [Fusion gene List compiled from FusionCatcher]

Babiceanu_Dataset

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The fusion gene pair WT1--EWSR1 information is not available in Babiceanu_Dataset.

Banned_Dataset

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Fusion gene WT1--EWSR1 is not found in the list of known false positive fusion genes. A candidate fusion gene found in this list has a very high probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

Known_Fusions

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Fusion gene WT1--EWSR1 has not been found in the list of fusions previously reported or published in scientific articles/reports/books/abstracts/databases, indexed by Google, Google Scholar, PubMed, etc. The list has been manually curated by FusionCatcher software. This label has only the role to answer with YES or NO the question "has ever before a given (candidate) fusion gene been published or reported?". This label does not have in anyway the role to provide the original references to the original scientific articles/reports/books/abstracts/databases for a given fusion gene.[Fusion gene List compiled from FusionCatcher]

ONGene Database

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The head gene WT1 is a known oncogene according to ONGENE database.


The tail gene EWSR1 is a known oncogene according to ONGENE database.

Bushman Cancer Gene Database

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The head gene WT1 is cancer associated according to Bushman Cancer Gene database.


The tail gene EWSR1 is cancer associated according to Bushman Cancer Gene database.

Tumor Gene Set By Uniprot

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The head gene WT1 is proto-oncogene or tumor suppresor gene according to Uniprot database.


The tail gene EWSR1 is proto-oncogene or tumor suppresor gene according to Uniprot database.

Oesophagus_Dataset

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Fusion gene WT1--EWSR1 is not found in oesophageal tumors from TCGA samples, which are published here.

Gliomas_Dataset

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Fusion gene WT1--EWSR1 is not found in the RNA-seq dataset of 272 glioblastomas, published here.

Prostate_Dataset

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The fusion gene pair WT1--EWSR1 information is not available in Prostate Dataset (150 prostate tumor RNAs, Robison et al, Integrative Clinical Genomics of Advanced Prostate Cancer, Cell, Vol. 161, May 2015, http://dx.doi.org/10.1016/j.cell.2015.05.001).

Pancreases_Dataset

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Fusion gene WT1--EWSR1 is not found in pancreatic tumor dataset, published here.

GTEx

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Fusion gene WT1--EWSR1 has not been found in a healthy sample (GTEx database of healthy tissues (thru FusionAnnotator)). A candidate fusion gene found in this set has a very high probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

Klijin_Dataset

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The fusion gene pair WT1--EWSR1 information is not available in Klijn Dataset.

Fimereli_Dataset

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The fusion gene pair WT1--EWSR1 information is not found in Fimereli_Dataset.

Literature

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The fusion gene pair WT1--EWSR1 information is not found in known fusion genelist compiled from literature.

Cortex_Dataset

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Fusion gene WT1--EWSR1 is not found in Cortex_Dataset (Fusion genes found in healthy human brains (BA9 prefrontal cortex)) . A candidate fusion gene found in this dataset has a very high probability of being a false positive.

ChromothripsisDB

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The fusion gene pair WT1--EWSR1 information is not available in ChromothripsisDB database.