NR4A3--EWSR1

Hyperlinks to databases below
COSMIC CHIMERKB CHIMERPUB CHIMERSEQ CHITARS
FARE-CAFE TICDB TUMOR_FUSION_GDPFusionCancerConjoinG
1000Genome18CancersBodymap2HPANon_Tumor_Cells
Babiceanu_DatasetBanned_DatasetKnown_FusionsONGene DatabaseBushman Cancer Gene Database
Tumor Gene Set By UniprotOesophagus_DatasetGliomas_DatasetProstate_DatasetPancreases_Dataset
GTExKlijn_DatasetFimereli_Dataset Literature Cortex_Dataset
ChromothripsisDB

Link to Genecards:

NR4A3 EWSR1

COSMIC

The fusion gene pair NR4A3--EWSR1 information is not available in COSMIC database.

ChimerKB

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The fusion gene pair NR4A3--EWSR1 information is available in CHIMERKB (CHIMERDB 3.0) database.

Fusion_pair5'Gene Junction (Chr/Position/Strand)3'Gene Junction (Chr/Position/Strand)Breakpoint_TypeGenome_BuildDiseaseValidationPMIDGene TypeSource
NR4A3_EWSR1-/727+404/ -/1614-251/ Exonic hg19 - - - Receptor; Transcription_Factor Cosmic_recurrent
NR4A3_EWSR1-/727+695/ -/1614-810/ Exonic hg19 - - - Receptor; Transcription_Factor Cosmic_recurrent
NR4A3_EWSR1-/727+655/ -/1632/ Exonic hg19 - - - Receptor; Transcription_Factor Cosmic_recurrent
NR4A3_EWSR1-/-/ -/-/ - hg19 chondrosarcoma - 12378528 Receptor; Transcription_Factor Cosmic_nonrecurrent
NR4A3_EWSR1-/-/ -/-/ - hg18 extraskeletal myxoid chondrosarcoma - 10359536 Receptor; Transcription_Factor OMIM
NR4A3_EWSR1-/-/ -/-/ - hg18 - - 1782363 Receptor; Transcription_Factor OMIM

ChimerPUB

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The fusion gene pair NR4A3--EWSR1 information is available in CHIMERPUB (CHIMERDB 3.0) database.

Fusion_pairTranslocationPMIDDiseaseValidationGene TypeSentence_highlight
NR4A3_EWSR1- 24746215chondrosarcoma;sarcoma FISH - Fluorescence in situ hybridization analysis showed EWSR1-NR4A3 gene fusion in 16 cases (62%), TAF15-NR4A3 gene fusion in 7 cases (27%), and TCF12-NR4A3 gene fusion in 1 case (4%). /// "In conclusion, EMCs with variant NR4A3 gene fusions show a higher incidence of rhabdoid phenotype, high-grade morphology, and a more aggressive outcome compared with the EWSR1-NR4A3 positive tumors. " /// "Extraskeletal myxoid chondrosarcomas (EMC) are rare soft tissue sarcomas with distinctive histology and uncertain histogenesis, characterized by Ewing sarcoma breakpoint region 1-nuclear receptor subfamily 4, group A, member 3 (EWSR1-NR4A3) fusion in 75% of the cases." /// " Extraskeletal myxoid chondrosarcomas (EMC) are rare soft tissue sarcomas with distinctive histology and uncertain histogenesis, characterized by Ewing sarcoma breakpoint region 1-nuclear receptor subfamily 4, group A, member 3 (EWSR1-NR4A3) fusion in 75% of the cases. "
NR4A3_EWSR1t(12;16)(q13;p11) / t(7;16)(q34;p11) / t(9;22)(q31;q12) / 18162764chondrosarcoma;liposarcoma;sarcoma FISH - Distinct chromosomal translocations have been identified in several myxoid sarcomas, including t(12;16)(q13;p11) FUS-DDIT3 in myxoid liposarcoma, t(7;16)(q34;p11) FUS-CREB3L2 in low-grade fibromyxoid sarcoma, and t(9;22)(q31;q12) EWSR1-NR4A3 in extraskeletal myxoid chondrosarcoma.
NR4A3_EWSR1t(9;22) (q22;q12) / 19775727chondrosarcoma;ewing sarcoma;sarcoma FISH - Most cases of EMC harbor the chromosomal reciprocal translocation t(9;22) (q22;q12) and the resultant fused gene, Ewing sarcoma region 1-nuclear receptor subfamily 4, group A, member 3 (EWSR1-NR4A3).
NR4A3_EWSR1t(9;22) / t(9;17) / t(9;15) / t(9;3) / 24508382chondrosarcoma;sarcoma FISH;PCR;RT-PCR - Extraskeletal myxoid chondrosarcoma is characterized by the reciprocal chromosomal translocation t(9;22) and the resultant fused gene EWS RNA-binding protein 1 and nuclear receptor subfamily 4, group A, member 3 (EWSR1-NR4A3). /// "The samples from 42 patients with extraskeletal myxoid chondrosarcoma were examined for the presence of EWSR1-NR4A3, TAF15-NR4A3, TCF12-NR4A3, and TFG-NR4A3 fusion transcripts by using RT-PCR. " /// "Of the 34 samples evaluable for fusion transcripts, 23 yielded positive results for EWSR1-NR4A3, 10 for TAF15-NR4A3, and 1 for TCF12-NR4A3. " /// "Of the 34 samples evaluable for fusion transcripts, 23 yielded positive results for EWSR1-NR4A3, 10 for TAF15-NR4A3, and 1 for TCF12-NR4A3."
NR4A3_EWSR1- 25693574epithelioid sarcoma FISH - The EWSR1 gene rearrangement was detected by fluorescence in situ hybridization (FISH), but molecular biology failed to detect EWSR1/ETS, EWSR1/NR4A3, EWSR1/DDIT3, EWSR1/ATF1, EWSR1-POU5F1, EWSR1/ZNF444, EWSR1-PBX1 gene fusions.
NR4A3_EWSR1- 22743288chondrosarcoma;synovial sarcoma;sarcoma FISH - Furthermore, reverse transcriptase-polymerase chain reaction studies of both the primary tumor and the recurrence confirmed the presence of both SS18-SSX2 and EWSR1-NR4A3 (exon 3) gene fusions, characteristic of SS and EMC, respectively. /// This is the first reported case of a remarkable soft tissue sarcoma that exhibits overlapping morphologic features between SS and EMC and that also harbors a combination of SS18-SSX2 and EWS-NR4A3 gene fusions.
NR4A3_EWSR1- 24703573chondrosarcoma;sarcoma FISH - Interestingly, all responsive cases turned out to express the typical EWSR1-NR4A3 fusion, while refractory cases carried the alternative TAF15-NR4A3 fusion. /// Genotype/phenotype analyses support a correlation between response and EWSR1-NR4A3 fusion.
NR4A3_EWSR1- 22567356dermatofibrosarcoma, - - Cytogenetic and molecular genetic analyses have shown that many of these sarcomas are characterized by recurrent chromosomal translocations resulting in highly specific fusion genes (e.g., FUS-DDIT3 in MLS, FUS-CREB3L2 in LGFMS, EWSR1-NR4A3 in EMC, and COL1A1-PDGFB in myxoid DFSP).
NR4A3_EWSR1- 18855877chondrosarcoma;sarcoma - - Bioinformatic analysis identified a DNA response element for EWSR1/NR4A3 in the PPARG promoter, and band-shift experiments and transient transfections indicate that EWSR1/NR4A3 can activate transcription through this element. /// "These results suggest that the overall expression of PPARG in EMC may be regulated in part by the balance between EWSR1/NR4A3 and NR4A3, and that PPARG may play a crucial role in the development of these tumours. " /// Western blot or immunohistochemical analyses confirm that PPARG and NDRG2 are expressed in tumours positive for EWSR1/NR4A3. /// The specific up-regulation of PPARG by EWSR1/NR4A3 may also have potential therapeutic implications.. /// The EWSR1/NR4A3 fusion protein of extraskeletal myxoid chondrosarcoma activates the PPARG nuclear receptor gene. /// "We performed an expression microarray analysis of EMC tumours expressing the EWSR1/NR4A3 fusion protein, comparing their expression profiles to those of other sarcoma types." /// "Western blots further show that an isoform of the native NR4A3 receptor lacking the C-terminal domain is very highly expressed in tumours positive for EWSR1/NR4A3, and co-transfections of this isoform along with EWSR1/NR4A3 indicate that it may negatively regulate the activity of the fusion protein on the PPARG promoter. " /// "We performed an expression microarray analysis of EMC tumours expressing the EWSR1/NR4A3 fusion protein, comparing their expression profiles to those of other sarcoma types. " /// The EWSR1/NR4A3 fusion protein of extraskeletal myxoid chondrosarcoma activates the PPARG nuclear receptor gene..
NR4A3_EWSR1- 23588370chondrosarcoma;sarcoma ISH - We propose to classify tumors with the appropriate phenotype and molecularly confirmed NR4A3/EWSR1 rearrangements as myxochondroid sarcoma, either osseous or extraskeletal variants..

ChimerSEQ

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The fusion gene pair NR4A3--EWSR1 information is not available in CHIMERSEQ (CHIMERDB 3.0) database.

ChiTaRS 2.1

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The fusion gene pair NR4A3--EWSR1 information is not available in CHITARS database.

FARE-CAFE

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The fusion gene pair NR4A3--EWSR1 information is available in FARE-CAFE.

Cancer Information
Fusion_ProteinCancer_TypeFP_Experimental_MethodFP_KaryotypeFP_Reference
NR4A3-EWSR1 Extra skeletal Myxoid Chondro sarcoma FISH,RT-PCR,PCR,DNA sequencing t(9;22)(q22;q11-12) PubMed:12378528

Genomic Information
Fusion_ProteinFusion_Protein_Isoforms5'_ProteinF_B_PFBP_in_Exon/Intron5'_Protein_on_Positive/Negative_Strands3'_ProteinS_B_P3'_Protein_on_Positive/Negative_StrandsSBP_in_Exon/IntronFusion_Protein_mRNA_sequenceFusion_Protein_ReferenceFusion_Protein_GenbankID
NR4A3-EWSR1 NR4A3-EWSR1 NR4A3 102588769 0 1 EWSR1 29693829 1 1 TCTAGCTAGAAACCTCAGAAAGACACAAATAAGAGCAGTAAAAaGGGAGCAAACTTAAAGTCTCCCTTGCTCGGAAAGAAGC 12378528 _

Domains and DDI Information
Fusion_ProteinFusion_Protein_IsoformsDomains_in_5'PartnerDDIs_for_Domains_in_5'PartnerDomains_in_3'PartnerDDIs_for_Domains_in_3'Partner5'_Protein5'P_Domains5'P_Domains_DDI_partners3'_Protein3'P_Domains3'P_Domains_DDI_partnersMissing_FP_DomainsMissing_FP_Domains_DDI
NR4A3-EWSR1 NR4A3-EWSR1 - - 1) zf-RanBP
1) ubiquitin , Ras
NR4A3 1) zf-C4
2) Hormone_recep
1) zf-C4 , Hormone_recep
2) zf-C4 , Hormone_recep , fn3 , Arm , SRC-1
EWSR1 1) RRM_6
2) zf-RanBP
1) RRM_6 , RRM_5 , RRM_1 , NUDIX_2 , Vinculin , zf-RNPHF
2) ubiquitin , Ras
1) zf-C4
2) Hormone_recep
3) RRM_6
1) zf-C4 , Hormone_recep
2) zf-C4 , Hormone_recep , fn3 , Arm , SRC-1
3) RRM_6 , RRM_5 , RRM_1 , NUDIX_2 , Vinculin , zf-RNPHF

miRNA Information
Fusion_ProteinmiRNAs_Targets_Fusion_proteinMissing_MiRNAs_Targets_Fusion_proteinFP_miRNA_Validation_method
NR4A3-EWSR1 hsa-let-7a-5phsa-miR-877-3phsa-miR-615-3phsa-miR-423-3phsa-miR-328-3phsa-miR-362-5phsa-miR-320ahsa-miR-149-5phsa-miR-23b-3phsa-miR-196a-5phsa-miR-100-5phsa-let-7c-5p hsa-miR-124-3phsa-miR-10b-5phsa-miR-26b-5p Report assay,Western Blot,Microarray,qPCR,NGS

Transcription Factors Information
Fusion_Protein5'P_Ref_mRNA_seqIDFP_Transcription_factorsFP_Missing_TFsFP_TF_Reference
NR4A3-EWSR1 NM_006981.3 1) HIFALPHA
2) TRIM28_HUMAN
3) MITF_HUMAN
4) STAT1_HUMAN
5) RB1_HUMAN
6) RBL2_HUMAN
7) E2F4_HUMAN
8) PRDM14_HUMAN
9) ETS1_HUMAN
10) EGR1_HUMAN
11) FOS_HUMAN
12) GATA3_HUMAN
13) E2F1_HUMAN
14) TAL1_HUMAN
15) CTCF_HUMAN
16) BRG1_HUMAN
17) HNF4A_HUMAN
18) TFAP2C_HUMAN
19) FOXA1_HUMAN
20) CEBPA_HUMAN
21) ESR1_HUMAN
22) ESR2_HUMAN
23) MEIS1_HUMAN
24) FOXP1_HUMAN
- 1) 20061373
2) 17542650
3) 21258399
4) 19122651
5) 20385362
6) 20385362
7) 21247883
8) 20953172
9) 20019798
10) 20690147
11) 20139302
12) 21878914
13) 21310950
14) 21795385
15) 21795385
16) 19505939
17) 21646355
18) 21572391
19) 21572391
20) 20219941
21) 21235772
22) 21235772
23) 21241896
24) 21924763

TicDB

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The fusion gene pair NR4A3--EWSR1 information is not available in TicDB.

TUMOR FUSION Gene Data Portal

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The fusion gene pair NR4A3--EWSR1 information is not available in TUMOR FUSION Gene Data Portal.

FusionCancer

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The fusion gene pair NR4A3--EWSR1 information is not available in FusionCancer Database.

ConjoinG

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The fusion gene pair NR4A3--EWSR1 information is not available in ConjoinG Database.

1000Genome

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Fusion gene NR4A3--EWSR1 has not been seen in a healthy sample (RNA-seq data from some samples from 1000 genomes project: Greger et al., Tandem RNA Chimeras Contribute to Transcriptome Diversity in Human Population and Are Associated with Intronic Genetic Variants, Plos One, Aug 2014 ). Therefore this candidate fusion gene has a low probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

18Cancers

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Fusion gene NR4A3--EWSR1 is not found in a RNA-seq dataset of 18 types of cancers from 600 tumor samples (B. Alaei-Mahabadia et al., Global analysis of somatic structural genomic alterations and their impact on gene expression in diverse human cancers, PNAS, Nov. 2016 )

Bodymap2

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Fusion gene NR4A3--EWSR1 is not found in the list of known false positive fusion genes. The list has been generated from healthy human samples collected from 16 organs from Illumina BodyMap2 RNA-seq database. A candidate fusion gene found in this list has a very high probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

HPA

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Fusion gene NR4A3--EWSR1 is not found in a healthy sample (RNA-seq database of 27 healthy tissues from 95 human individuals). A candidate fusion gene found in this dataset has a very high probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

Non_Tumor_Cells

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Fusion gene NR4A3--EWSR1 was not found among the fusion genes which have been previously reported/found in non-tumor cell lines, like for example HEK293. The genes which are observed in those list can be considered as non-somatic mutation. [Fusion gene List compiled from FusionCatcher]

Babiceanu_Dataset

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The fusion gene pair NR4A3--EWSR1 information is not available in Babiceanu_Dataset.

Banned_Dataset

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Fusion gene NR4A3--EWSR1 is not found in the list of known false positive fusion genes. A candidate fusion gene found in this list has a very high probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

Known_Fusions

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Fusion gene NR4A3--EWSR1 has not been found in the list of fusions previously reported or published in scientific articles/reports/books/abstracts/databases, indexed by Google, Google Scholar, PubMed, etc. The list has been manually curated by FusionCatcher software. This label has only the role to answer with YES or NO the question "has ever before a given (candidate) fusion gene been published or reported?". This label does not have in anyway the role to provide the original references to the original scientific articles/reports/books/abstracts/databases for a given fusion gene.[Fusion gene List compiled from FusionCatcher]

ONGene Database

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The head gene NR4A3 is not a known oncogene according to ONGENE database.


The tail gene EWSR1 is a known oncogene according to ONGENE database.

Bushman Cancer Gene Database

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The head gene NR4A3 is cancer associated according to Bushman Cancer Gene database.


The tail gene EWSR1 is cancer associated according to Bushman Cancer Gene database.

Tumor Gene Set By Uniprot

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The head gene NR4A3 is proto-oncogene or tumor suppresor gene according to Uniprot database.


The tail gene EWSR1 is proto-oncogene or tumor suppresor gene according to Uniprot database.

Oesophagus_Dataset

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Fusion gene NR4A3--EWSR1 is not found in oesophageal tumors from TCGA samples, which are published here.

Gliomas_Dataset

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Fusion gene NR4A3--EWSR1 is not found in the RNA-seq dataset of 272 glioblastomas, published here.

Prostate_Dataset

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The fusion gene pair NR4A3--EWSR1 information is not available in Prostate Dataset (150 prostate tumor RNAs, Robison et al, Integrative Clinical Genomics of Advanced Prostate Cancer, Cell, Vol. 161, May 2015, http://dx.doi.org/10.1016/j.cell.2015.05.001).

Pancreases_Dataset

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Fusion gene NR4A3--EWSR1 is not found in pancreatic tumor dataset, published here.

GTEx

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Fusion gene NR4A3--EWSR1 has not been found in a healthy sample (GTEx database of healthy tissues (thru FusionAnnotator)). A candidate fusion gene found in this set has a very high probability of being a false positive. [Fusion gene List compiled from FusionCatcher]

Klijin_Dataset

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The fusion gene pair NR4A3--EWSR1 information is not available in Klijn Dataset.

Fimereli_Dataset

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The fusion gene pair NR4A3--EWSR1 information is not found in Fimereli_Dataset.

Literature

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The fusion gene pair NR4A3--EWSR1 information is not found in known fusion genelist compiled from literature.

Cortex_Dataset

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Fusion gene NR4A3--EWSR1 is not found in Cortex_Dataset (Fusion genes found in healthy human brains (BA9 prefrontal cortex)) . A candidate fusion gene found in this dataset has a very high probability of being a false positive.

ChromothripsisDB

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The fusion gene pair NR4A3--EWSR1 information is not available in ChromothripsisDB database.