| MDM2_CDK4 | - |
17895748 | dedifferentiated liposarcoma |
FISH;PCR |
- |
To evaluate the accuracy of fluorescence in situ hybridization (FISH) analysis in the differential diagnosis of adipose tissue tumors, we investigated MDM2-CDK4 status by FISH, real-time polymerase chain reaction (PCR) [quantitative PCR (Q-PCR)] and immunohistochemistry (IHC) in a series of 200 adipose tumors. /// "In conclusion, we recommend that the evaluation of MDM2-CDK4 amplification using FISH or Q-PCR be used to supplement IHC analysis when diagnosis of adipose tissue tumors is not possible based on clinical and histologic information alone.. " /// Detection of MDM2-CDK4 amplification by fluorescence in situ hybridization in 200 paraffin-embedded tumor samples: utility in diagnosing adipocytic lesions and comparison with immunohistochemistry and real-time PCR.. /// "First, we evaluated MDM2-CDK4 amplification and expression in a series of 94 well-defined adipose tissue tumors. "
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| MDM2_CDK4 | - |
25679065 | liposarcoma;sarcoma |
FISH;PCR |
- |
Four cases of atypical lipomatous tumor/well-differentiated liposarcoma (4/26, 15%) with a low MDM2 and CDK4 amplification level (MDM2-CDK4/CEP12 ratio ranging between 2 and 2.5) detected by FISH showed no amplification by MLPA, although gain of MDM2 and CDK4 (ratios ranging between 1.6 and 1.9) was seen with MLPA. /// "Detection of MDM2/CDK4 amplification in lipomatous soft tissue tumors from formalin-fixed, paraffin-embedded tissue: comparison of multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH).. " /// "In conclusion, MLPA proves to be an appropriate and straightforward technique for screening MDM2/CDK4 amplification in lipomatous tumors, especially when a correct cut-off value and reference samples are chosen, and could be considered a good alternative to FISH to determine MDM2 and CDK4 amplification in liposarcomas. " /// "In case of high-level amplification (MDM2-CDK4/CEP12 ratio >5), concordance was 100%. "
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| MDM2_CDK4 | - |
10755400 | liposarcoma;sarcoma |
FISH |
- |
All lipomas displayed a null MDM2/CDK4 phenotype, whereas all LL-WDL showed MDM2/CDK4 or CDK4 phenotypes. /// The expression of MDM2/CDK4 gene product in the differential diagnosis of well differentiated liposarcoma and large deep-seated lipoma.. /// "In conclusion, MDM2/CDK4 product-based immunophenotyping appears to represent a valuable method for the categorization of arguable LDSL.. "
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| MDM2_CDK4 | - |
24557201 | liposarcoma;sarcoma |
FISH |
- |
MDM2/CDK4 gene amplification has been shown to be sensitive and specific for the diagnosis of well-differentiated lipoma-like liposarcoma (WD-LLLPS). /// "The incidence of MDM2/CDK4 amplification was reassuringly low (5%) in this cohort of LN, despite their large and/or deep-seated nature. " /// Two (3%) cases showed dual MDM2/CDK4 amplification and 2 (3%) showed MDM2 amplification alone. /// Mdm2/Cdk4 amplification in large and/or deep-seated lipomatous neoplasms without overtly atypical morphological features.. /// "The clinical, radiological and pathological features of all cases with CDK4/MDM2 amplification were reviewed. " /// To assess the incidence of MDM2/CDK4 amplification in large and/or deep seated LN without overtly atypical morphological features and to examine the clinicopathological features of cases showing these alterations.
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| MDM2_CDK4 | - |
20228779 | liposarcoma;sarcoma |
FISH |
- |
FISH analysis revealed a deletion of the Rb-1 gene in all six cases, whereas no MDM2/CDK4 amplification was identified in all cases tested.
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| MDM2_CDK4 | - |
24608573 | breast cancer |
- |
- |
There was an increased frequency of CDK4/MDM2 amplifications in recurrences, as well as gains and losses of other actionable alterations.
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| MDM2_CDK4 | - |
20834238 | benign fibrous histiocytoma |
- |
- |
It is less sensitive than MDM2/CDK4 for dedifferentiated liposarcomas diagnosis, but it appears more specific to distinguish dedifferentiated liposarcomas from other poorly differentiated sarcomas.
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